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KMID : 0363320210420061223
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Journal of Korean Oriental Internal Medicine
2021 Volume.42 No. 6 p.1223 ~ p.1236
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Effect of Sosiho-tang on a Thioacetamide-induced Liver Fibrosis Mouse Model
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Lee Se-Hui
Oh Min-Hyuck
Shin Mi-Rae
Lee Ji-Hye
Roh Seong-Soo
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Abstract
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Objectives: The aim of the current study was to investigate the effect of Sosiho-tang on thioacetamide (TAA)-induced liver fibrosis in mice and to elucidate its underlying mechanisms.
Methods: The mice were divided into 4 groups: Normal mice (Normal), TAA-induced control mice (Control), TAA-induced and silymarin-treated (50 mg/kg) mice (Silymarin), and TAA-induced and Sosiho-tang treated (200 mg/kg) mice (SSHT). Liver fibrosis was induced via intraperitoneal injection of TAA three times a week for 8 weeks. Silymarin and Sosiho-tang were concomitantly administered for 8 weeks. Serum and liver tissues were then collected and the anti-oxidant and inflammatory protein levels in the liver tissues were evaluated using western blotting.
Results: SSHT administration significantly reduced the levels of AST, ALT, ammonia, and MPO in the serum. SSHT also significantly down-regulated liver NADPH oxidase and regulated the Nrf2/Keap1 signaling pathway. SSHT treatment downregulated the liver NF-κB levels and suppressed inflammatory cytokines. SSHT treatment also decreased bile acid-related factors, such as CYP7A1 and NTCP, and fibrosis-related factors, such as α-SMA and Collagen Ⅰ.
Conclusions: Taken together, these data suggest that SSHT administration suppressed the progression of liver fibrosis by activating the Nrf2/Keap1 pathway and inhibiting NF-κB.
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KEYWORD
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Sosiho-tang, Xiao-chai-hu-tang, liver fibrosis, thioacetamide, anti-inflammatory
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